medicinal plant extract concentration

medicinal plant extract concentration

Total phenolics concentration and antioxidant potential of ...

Thirty-seven plant organs, traditionally used as drugs, collected in Pakistan, were extracted with 70% acetone and analyzed for their total phenolics concentration and antioxidant potential. Seven extracts showed more than 85% inhibition of lipid peroxidation in vitro as compared with blank.

More

Relative efficacy of three medicinal plant extracts in the ...

On the other hand, T(4) concentration was increased by B. monnieri extract suggesting its thyroid-stimulating role. When the relative potency of each plant extract was calculated in terms of percent increase or decrease in thyroid hormones, as compared to the control value, the decrease in T(3) concentration by A. marmelos was about 62% ...

More

Medicinal Plant Leaf Extract and Pure Flavonoid Mediated ...

Nov 20, 2017  The effect of concentration of plant extract and quercetin on the formation of AgNPs was studied systematically by mixing 2 mM AgNO 3 solution and Tulsi extract in different concentration

More

Protocol: Extract bioactive substances from plants – a ...

threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' – the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

More

Effect of medicinal plant type and concentration on ...

Three medicinal plants, namely, cinnamon (CIN), cardamom (CAR), and Shirazi thyme (SHT) were applied to the kombucha medium to evaluate the effects of medicinal plants on the physicochemical, antioxidant, and sensorial properties of final kombucha samples, and then the kombucha was mixed with green tea at a ratio of 50:50 (0.35%, w/w: 0.35%, w ...

More

TOTAL PHENOLIC CONTENT, FLAVONOID CONCENTRATION

Determination of flavonoid concentrations in the plant extracts The content of flavonoids in the examined plant extracts was determined using spectrophotometric method (QUETTIER et al., 2000). The sample contained 1 ml of methanol solution of the extract in the concentration of 1 mg/ml and 1 ml of 2% AlCl3 solution dissolved in methanol.

More

Relative efficacy of three medicinal plant extracts in the ...

Jul 01, 2002  When relative potencies of the three plant extracts with respect to the control values were calculated, maximum inhibition in T 3 concentration was observed by A. marmelos leaf extracts (61.78%), followed by A. vera (25.13%), while T 4 was inhibited only by 12.88% by the latter one.B. monnieri and A. marmelos could enhance the T 4 concentrations by 41.05 and 36.22%, respectively.

More

(PDF) Plant...Extraction - ResearchGate

Minimum inhibitory concentration (MIC) of the extracts, fractions, or purifie d . compounds can be assessed by micro-dilution assay. ... Journal of Medicinal Plants Research V ol. 4(2), ...

More

Antimicrobial activity of some plant extracts against ...

Feb 01, 2018  All plant extracts was potentially effective against S. typhi, E. coli, B. cereus and S. aureus implicated in food spoilage but the extract of Punica granatum was the most effective extract with concentration of 500 mg/ml. Ethanolic P. granatum peels extracts was found to be potentially effective against Micrococcus luteus, S. aureus, Bacillus ...

More

Protocol: Extract bioactive substances from plants – a ...

threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' – the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

More

Effect of medicinal plant type and concentration on ...

Three medicinal plants, namely, cinnamon (CIN), cardamom (CAR), and Shirazi thyme (SHT) were applied to the kombucha medium to evaluate the effects of medicinal plants on the physicochemical, antioxidant, and sensorial properties of final kombucha samples, and then the kombucha was mixed with green tea at a ratio of 50:50 (0.35%, w/w: 0.35%, w ...

More

TOTAL PHENOLIC CONTENT, FLAVONOID

Determination of flavonoid concentrations in the plant extracts The content of flavonoids in the examined plant extracts was determined using spectrophotometric method (QUETTIER et al., 2000). The sample contained 1 ml of methanol solution of the extract in the concentration of 1 mg/ml and 1 ml of 2% AlCl3 solution dissolved in methanol.

More

Extraction and phytochemical analysis of medicinal plants

Extraction and phytochemical analysis of medicinal plants ... Total phenol content % of phenol content µg GAE/µg Concentration (µg/ml) Hexane extract Acetone extract Methanol extract Water extract 100 15.69 18.5 25.6 22.8 200 22.54 26.9 45.86 41.8 300 30.41 38.9 72.8 68.4 400 42.89 54.78 88.96 76.5 500 54.58 65.8 105.68 95.8 Figure 4. Total ...

More

ANTIMICROBIAL ACTIVITY OF SOME MEDICINAL PLANT

flavonoids, saponins, tannins, and terpenoids were found in one or more of the plant extracts, and all the plant extracts demonstrated scavenging power against DPPH.The cytotoxicity of extracts of Garcinia livingstonei, and Sclerocarya birrea barks ranged between

More

Evaluation of antimicrobial activity of different solvent ...

Stock solution of each plant extract was prepared at a concentration of 1 mg/ml in different plant extacts viz. Methanol, Ethanol, Petroleum Ether, Water. About 100 µl of different concentrations of plant solvent extracts were added sterile syringe into the wells and allowed to diffuse at room temperature for 2hrs. Control experiments ...

More

Minimum Inhibitory Concentration (MIC) of antimicrobial ...

Jul 04, 2017  Minimum Inhibitory Concentration (MIC) of antimicrobial activity in various dried extracts of Gnaphalium polycaulon, Indian folkloric medicinal plant K. Shanmugapriya*, T. Senthil Murugan and Tha Thayumanavan School of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore - 641014, Tamil Nadu, India.

More

Cytotoxicity Evaluation of Methanol Extracts of Some ...

A stock solution of each plant extracts was prepared by dissolving 30 mg of extract in 95 μl of dimethyl-sulfoxide (DMSO, Merck, Germany) and 2905 μl of cell culture medium to a final stock concentration of 10mg/ml and then diluted with complete culture medium to reach the desired concentrations. For

More

Antimicrobial Activity of Medicinal Plants against Human ...

A wide range of medicinal plant parts is used for extract as raw drugs and they posses varied medicinal properties (Srivastav et. al., 1996). Medicinal plants are believed to be important source of new chemical substance s with potential therapeutic effects (Katrin Basha et. al., 2011). The secondary metabolites of plants were found to be

More

In Vitro Screening for Cytotoxic Activity of Herbal Extracts

Many commercially sold medicinal plants might contain chemical substances with potential mutagenic and/or carcinogenic properties [6, 7] as well as with antitumor properties [8, 9], and the active extracts detected by screening methods should be subjected to accurate bioassays to determine their specific pharmacological activity.

More

RESEARCH ANTIFUNGAL ACTIVITY OF MEDICINAL PLANT

minimal inhibitory concentration (MIC) values of plant extracts. Plant extracts were serially diluted, ranging from 1/2 up to a 1/100 dilution from the crude extract. In each well, 100 µL of each extract dilution was mixed with 100 Table 1. List of medicinal plants used in this work with the popular uses in Uruguay. Lonicera japonica Thunb.

More

Comparison of Different Solvents for Phytochemical ...

Background: The bioactive components present in the Datura metel plant are known to be responsible for its medicinal properties, to cure many diseases like asthma and bronchitis. However, the extraction method for these bioactive components is not yet standardized. The present study was undertaken to compare the effect of using different extraction solvents to extract the active components ...

More

Evaluation of Antibacterial Activity of Some Traditionally ...

2. Materials and Methods 2.1. Plant Materials and Extract Preparation. Popular plants used in traditional medicine by the Nepalese people across the country, based upon previous ethnobotanical literatures and potential medicinal values as judged by local healers, were screened and selected to

More

Frontiers Antimicrobial Properties and Mechanism of ...

Jul 24, 2018  Medicinal plants contain several phytochemicals such as flavonoids, alkaloids, tannins, and terpenoids, ... Stained cells were aliquoted into control, treatment groups and cell-free extracts. 1 ml of plant extract at a concentration of 20% was added to 1 ml of bacteria or cell-free filtrate. For control groups, 1 ml of PP buffer was added to 1 ...

More

Protocol: Extract bioactive substances from plants – a ...

threat. A lot of plants contain toxic compounds. Even with low concentration of compounds occurring in the original plant material, be aware of ''sola dosis facit venenum'' – the dose makes the poison. Sometimes an extract or even the working process can be extremly dangerous due to the high concentration of substance.

More

Effect of medicinal plant type and concentration on ...

Three medicinal plants, namely, cinnamon (CIN), cardamom (CAR), and Shirazi thyme (SHT) were applied to the kombucha medium to evaluate the effects of medicinal plants on the physicochemical, antioxidant, and sensorial properties of final kombucha samples, and then the kombucha was mixed with green tea at a ratio of 50:50 (0.35%, w/w: 0.35%, w ...

More

Extraction and phytochemical analysis of medicinal plants

Extraction and phytochemical analysis of medicinal plants ... Total phenol content % of phenol content µg GAE/µg Concentration (µg/ml) Hexane extract Acetone extract Methanol extract Water extract 100 15.69 18.5 25.6 22.8 200 22.54 26.9 45.86 41.8 300 30.41 38.9 72.8 68.4 400 42.89 54.78 88.96 76.5 500 54.58 65.8 105.68 95.8 Figure 4. Total ...

More

RESEARCH ANTIFUNGAL ACTIVITY OF MEDICINAL PLANT

minimal inhibitory concentration (MIC) values of plant extracts. Plant extracts were serially diluted, ranging from 1/2 up to a 1/100 dilution from the crude extract. In each well, 100 µL of each extract dilution was mixed with 100 Table 1. List of medicinal plants used in this work with the popular uses in Uruguay. Lonicera japonica Thunb.

More

Artemisia plant extracts show potential anti-SARS-CoV-2 ...

Feb 16, 2021  Their findings reveal that various extracts of two medicinal plants, ... cell line revealed that the extracts at a concentration of less than 2 mg/ml had potent antiviral activity against SARS-CoV ...

More

Cytotoxicity Evaluation of Methanol Extracts of Some ...

A stock solution of each plant extracts was prepared by dissolving 30 mg of extract in 95 μl of dimethyl-sulfoxide (DMSO, Merck, Germany) and 2905 μl of cell culture medium to a final stock concentration of 10mg/ml and then diluted with complete culture medium to reach the desired concentrations. For

More

Antimicrobial Activity of Medicinal Plants against Human ...

A wide range of medicinal plant parts is used for extract as raw drugs and they posses varied medicinal properties (Srivastav et. al., 1996). Medicinal plants are believed to be important source of new chemical substance s with potential therapeutic effects (Katrin Basha et. al., 2011). The secondary metabolites of plants were found to be

More

Antimicrobial activity of crude extracts of three ...

15 Afr. J. Trad. CAM (2006) 3 (4): 13 - 22 medicinal plants claimed by traditional practitioners to cure some ailments of microbial origins (Akinyemi et al., 2005 ; Ajaiyeoba,2002; llori ., 1996; Akinsinde and et al Olukoya ,1995). In recent times , persistent claimed of efficacy of the crude extracts of A. wilkesiana, P. discoideus, T. guineensis when used separately in the treatment of

More

Comparison of Different Solvents for Phytochemical ...

Background: The bioactive components present in the Datura metel plant are known to be responsible for its medicinal properties, to cure many diseases like asthma and bronchitis. However, the extraction method for these bioactive components is not yet standardized. The present study was undertaken to compare the effect of using different extraction solvents to extract the active components ...

More

Antibacterial activity of plant extracts from northwestern ...

higher plants are an interesting source of antibacterial agents to be applied for external use, e.g. cataplasms, compresses, gargles, and ointments. The purpose of our research was the determination of the antibacterial activity of aqueous and ethanolic extracts of 12 northwestern Argentinian plants

More

Annex 1 - WHO

3.5 Concentration and drying 115 3.6 Fermentation 116 ... (GACP) for medicinal plants (1) cover the cultivation and collection of medicinal plants, together with certain post-harvest operations in which the concept of ... obtained include extracts, decoctions, tinctures, essential oils and others. The

More

ISSN Phytochemical Analysis and Free Radical Scavenging ...

The extracts were diluted with distilled water to a known concentration in order to obtain the readings within the standard curve range of 0.0 to 600 µg of tannic acid/ml. 250 µl of diluted extract or tannic acid solution was mixed with 1 ml of distilled water in a test tube followed by the addition of 250 µl of Folin - Ciocalteu reagent.

More

(PDF) Ethnomedicinal and phytochemical review of Pakistani ...

Mostly methanolic extracts of medicinal plants were used in different studies, which have shown comparatively higher inhibitory activities against E. coli than n-hexane and aqueous extracts.

More

In vitro antimicrobial evaluation of whole-plant extracts ...

The Minimum Inhibitory Concentr ~ 101 ~ Journal of Medicinal Plants Studies ations (MIC) values of the extracts are shown in Table 2. Against Staphylococcus aureus, the minimum inhibitory concentration for both ethyl acetate and chloroformv was 100 mg/ml.

More